Malays. Appl. Biol. (2009) 38(1): 61–64
CHROMOSOME ANALYSIS OF WALKING CATFISH, Clarias spp.
SITI SHAPOR SIRAJ1, RATIAH SUKARDI1, CHERYL CHEAH PHAIK IMM1, KHEW HUAI MEI1, SHALINI VELLASAMY1, JOTHI MALAR PANANDAM2 and SITI KHALIJAH DAUD3
1Department of Aquaculture, Faculty of Agriculture,
2Department of Animal Science, Faculty of Agriculture,
3Department of Biology, Faculty of Science,
Universiti Putra Malaysia, 43400 Serdang, Selangor
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ABSTRACT
Karyotyping is one of the useful tools for species identification, taxonomy, evolutionary and breeding selection. To date, no information on karyotyping of this fish in the country is documented. The aim of this study was to determine and differentiate the karyotypic structure and fundamental arm number of the walking catfish; Clarias macrocephalus, Clarias batrachus and Clarias gariepinus. The fish samples ranging from 23–160gm in weight and 16–40cm in length were used to obtain the chromosomes by subjecting to a combination of colchicine and phytohaemagglutinin for arresting a mitotic division. The karyotype of each species was arranged according to size, type and structure such as metacentric (M), submetacentric (SM), subtelocentric (ST) and acrocentric/telocentric (A/T). The diploid chromosome numbers ranged from 2n equal to 54 (Clarias macrocephalusand Clarias batrachus) to 2n equal to 56 (Clarias gariepinus).
ABSTRAK
Kajian kariotip merupakan salah satu kaedah penting untuk pengenalpastian spesies, taksonomi, evolusi dan pemilihan baka. Hingga kini tiada maklumat kariotip ikan ini didokumentasikan di negara. Tujuan kajian adalah untuk menentukan dan membezakan struktur kariotip dan nombor lengan asas ikan keli; Clarias macrocephalus, Clarias batrachusdan Clarias gariepinus. Sampel ikan yang mempunyai julat berat 23–160gm dan julat panjang 16–40cm digunakan untuk memperoleh kromosom melalui suntikan yang mengandungi kolchisina dan fitohemaglutinin bagi menghalang pembahagian mitosis. Kariotip setiap spesies disusun mengikut saiz, jenis dan struktur seperti metasentrik (M), submetasentrik (SM), subtelosentrik (ST) dan akrosentrik/telosentrik (A/T). Bilangan kromosom diploid berjulat di antara 2n = 54 (Clarias macrocephalus danClarias batrachus) hingga 2n = 56 (Clarias gariepinus).
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