44_3_11

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Malays. Appl. Biol. (2015) 44(3): 55-62

 

COMPARATIVE STUDY ON MEMBRANE SOLUBILISATION OF BIOSYNTHESIZED NANO-SILVER & BIOSYNTHESIZED NANO-ZINC OXIDE ON SELECTED SPERM PARAMETER

NUR HILWANI ISMAIL, MOHAMMAD LUQMAN ZULKIPLI, HELMI KHUSAIRI BAHARUDIN, NURUL HUSNA RIFIN AND RABIATULADAWIYAH MD. AKHIR

School of Biological Sciences, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

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ABSTRACT

Extensive applications of nanoparticles are used in the field of catalysis, biosensing, imaging, drug delivery, nano-device fabrication and medicine. Previously, biosynthesized nano-silver and biosynthesized nano-zinc oxide has shown significant effect as antimicrobial agents, thus reduces concern over the threat of antibiotic resistance. Biosynthesis of nano-silver and nano-zinc oxide using Pandanus amaryllifolius leaf extract and their spermicidal effect was explored in the present research following characterization using FESEM-EDX and were determined to be spherical in shape and aggregated into regular structure with high uniformity.  The sperm membrane solubilisation property displayed by both biosynthesized nano-silver and biosynthesized nano-zinc oxide exceeded the effects of 1% (w/v) Triton-X and was most significant at the concentration of 100 µg/ml. The results suggested that biosynthesized nano-silver and biosynthesized nano-zinc oxide holds possibility as new generation spermicidal agents.

Keywords

Sperm membrane, cytotoxicity, nanoparticles, green synthesis, FESEM